2007年度

Engineering, expression, purification, and production of recombinant thermolysin.
Inouye K, Kusano M, Hashida Y, Minoda M, Yasukawa K (2007) Biotechnol. Ann. Rev. 13: 43-64


Improving the activity and stability of thermolysin by site-directed mutagenesis.
Yasukawa K, Inouye K (2007) Biochim. Biophys. Acta. 1774: 1281-1288


Improvement in performance of affinity gels containing Gly-D-Phe as a ligand to thermolysin due to increasing the spacer chain length.
Inouye K, Nakamura K, Kusano M, Yasukawa K (2007) Biosci. Biotechnol. Biochem. 71: 2083-2086


Effects of Neutral Salts and Alcohols on the Activity of Streptomyces caespitosus Neutral Protease (ScNP).
Inouye K, Shimada T, Yasukawa K (2007) J .Biochem. 142: 317-324


Purification to Homogeneity of a Neutral Metalloproteinase from Streptomyces caespitosus.
Inouye K, Shimada T, Yasukawa K (2007) Biosci. Biotechnol. Biochem. 71: 1773-1776


A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli.
Yasukawa K, Kusano M, Inouye K (2007) Protein Eng. Des. Sel. 20: 375-383


Kinetic Analysis of the Activation-and-Inhibition Dual Effects of Cobalt Ion on Thermolysin Activity.
Hashida Y, Inouye K (2007) J .Biochem. 141: 843-853


Molecular Mechanism of the Inhibitory Effect of Cobalt Ion on Thermolysin Activity and the Suppressive Effect of Calcium Ion on the Cobalt Ion-dependent Inactivation of Thermolysin.
Hashida Y, Inouye K (2007) J .Biochem. 141: 879-888


Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 of thermolysin on its catalytic activity.
Tatsumi C, Hashida Y, Yasukawa K, Inouye K (2007) J .Biochem. 141: 835-842