2010年度

Effects of organic solvents on thereverse transcriptase reaction catalyzed by reverse transcriptases from avianmyeloblastosis virus and Moloney murine leukaemia virus.
Yasukawa K, Konishi A, Inouye K (2010) Biosci. Biotechnol. Biochem. 74 : 1925-1930

Effects of the mutational combinations on the activity and stability ofthermolysin.
Kusano M, Yasukawa K, Inouye K (2010) J. Biotechnol. 147: 7-16

Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification.
Yasukawa K, Agata N, Inouye K (2010) Enzyme Microb. Technol. 46: 391-396

Synthesis of N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester catalyzed by thermolysin variants with improved activity.
Kusano M, Yasukawa K, Inouye K (2010) Enzyme Microb. Technol. 46: 320-325

Insight into the mechanism of the stabilization of Moloney murine leukaemiavirus reverse transcriptase.
Mizuno M, Yasukawa K, Inouye K (2010) Biosci. Biotechnol. Biochem. 74: 440-442

Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1.
Tsukiyama T, Lee J, Okumoto Y, Teraishi M, Tanisaka T, Inouye K (2010)Biosci. Biotechnol. Biochem. 74: 430-432

The optimal activity of a pseudozymogenform of recombinant matriptase under the mildly acidic pH and low ionicstrength conditions.
Inouye K, Yasumoto M, Tsuzuki S, Mochida S, Fushiki T (2010) J. Biochem. 147: 485-492

Kinetic analysis and mechanism on the inhibition of chlorogenic acid andits components against porcine pancreas α-amylase isozymes I and II.
Narita Y, Inouye K (2010) J. Agric. Food Chem. 57: 9218-9225


The role of asparagine-linkedglycosylation site on the catalytic domain of matriptase in its zymogenactivation.
Miyake Y, Yasumoto M, Tsuzuki S, Fushiki T, Inouye K (2010) Biochim. Biophys. Acta 1804: 156-165


Matriptrase dose not require hepatocyte growth factor activator inhibitortype-1 for activation in an epithelical cell expression model.
Miyake Y, Tsuzuki S, Fushiki T, Inouye K (2010) Biosci. Biotechnol. Biochem. 74: 848-850


Effects of site-directed mutagenesis of the loop residue of the N-terminaldomain of Gly117 of thermolysin on its catalytic activity.
Menach E, Yasukawa K, Inouye K (2010) Biosci. Biotechnol. Biochem. 74: 2457-2462


Expression in Escherichia coli, refolding, and purification of the recombinant mature form of human matrix metalloproteinase 7 (MMP-7).
Muta Y, Yasui N, Matsumiya Y, Kubo M, Inouye K (2010) Biosci. Biotechnol. Biochem. 74: 2515-2517


The structural requirementsof matriptase in its ectodomain release in polarized epithelial cells
Tsuzuki S, Murai N, Miyake Y, Inouye K, Fushiki T (2010) Biosci. Biotechnol. Biochem. 74: 1295-1297


Identification of the matriptase second CUB domain as the secondary sitefor interaction with hepatocyte growth factor activator inhibitor type-1.
Inouye K, Tsuzuki S, Yasumoto M, Kojima K, Mochida S, Fushiki T (2010)J. Biol. Chem. 285: 33394-33403


Increase in thermal stability of Moloney murine leukaemia virus reversetranscriptase by site-directed mutagenesis.
Yasukawa K, Mizuno M, Konishi A, Inouye K (2010) J. Biotechnol. 150: 299-306