2006年度

Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin
Yasukawa K, Kusano M, Nakamura K, and Inouye K (2006) Protein Expr Purif 46: 332-336


Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization
Inouye K, Minoda M, Takita T, Sakurama H, Hashida Y, Kusano M, Yasukawa K (2006) Protein Expr Purif 46: 248-255


Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats
Hamamoto H, Kusudo T, Urushino N, Masuno H, Yamamoto K, Yamada S, Kamakura M, Ohta M, Inouye K, and Sakaki T (2006) Molecular Pharmacology 70: 120-128


Purification and some properties of a D-fructose-1,6-bisphosphate aldolase from red alga, Gracilaria chorde Holmes
Kakita H, Kamishima H, and Inouye K (2006) Botanica Marina 49: 174-181


Interaction between mitochondrial CYP27B1 and adrenodoxin: role of arginine 458 of mouse CYP27B1
Urushino M, Yamamoto K, Kagawa N, Ikushiro S, Kamakura M, Yamada S, Kato S, Inouye K, and Sakaki T (2006) Biochemistry 45: 4405-4412


Enzymatic properties of human CYP2W1 expressed in Escherichia coli
Yoshioka H, Kasai N, Ikushiro S, Shinkyo R, Kamakura M, Ohta M, Inouye K, and Sakaki T (2006) Biophys Biochem Res Commun 345: 169-174


Biodegradation of dioxins by recombinant Escherichia coli. expressing rat CYP1A1 or its mutant
Shinkyo R, Kamakura M, Ikushiro S, Inouye K, and Sakaki T (2006) Appl Microbiol Biotechnol 72: 584-590


Uronic acid determination by high performance liquid chromatography with postcolumn fluorescence derivatization
Kakita H, Kamishima H, and Inouye K (2006) J Chromatogr A 1129: 296-299


Engineering of the pH-dependence of thermolysin activity as examined by site-directed mutagenesis of Asn112 located at the active site of thermolysin
Kusano M, Yasukawa K, Hashida Y, and Inouye K (2006) J Biochem 139: 1017-1023


Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis a-amylases and insights into engineering a-amylase variants active under acidic conditions
Lee S, Oneda H, Minoda M, Tanaka A, and Inouye K (2006) J Biochem 139: 997-1005


Comparison of the wild-type a-amylase and its variants enzymes in Bacillus amyloliquefaciens in activity and thermal stability, and insights into engineering the thermal stability of Bacillus a-amylase
Lee S, Mouri Y, Minoda M, Oneda H, and Inouye K (2006) J Biochem 139:1007-1015